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e-ased.org, vol. 33, no. 2, pp.140-143, 2017

DOI: https://doi.org/10.5635/ASED.2017.33.2.010

Development and Characterization of 14 Microsatellite Markers for the Antarctic Midge Parochlus steinenii (Diptera, Chironomidae) in Maritime Antarctic

Hanna Kim1,#, Seunghyun Kang2,#, Hanul Kim1, Sanghee Kim3, Jongwoo Jung1,4,*

Affiliation: 1The Division of EcoCreative, Ewha Womans University, Seoul 03760, Korea 2Unit of Polar Genomics, Korea Polar Research Institute, Incheon 21900, Korea 3Division of Polar Life Sciences, Korea Polar Research Institute, Incheon 21900, Korea 4Department of Science Education, Ewha Womans University, Seoul 03760, Korea #These authors contributed equally to this work.

Abstract: A winged midge species, Parochlus steinenii is one of the most abundant species in Antarctica, which is distributed over a wide area from the South American continent to the South Shetland Islands in Antarctica. It was dispersed into islands in the South Shetland Islands from the South American continent, and it adapted to a variety of environments and settled. This species, therefore, is a good model organism to explain the evolutionary process of Antarctic terrestrial fauna. Nevertheless, there are few genetic studies on this species, which are necessary for understanding the genetic diversity, population structure, etc. Here, we developed and characterized 14 polymorphic microsatellite markers. The number of alleles per locus ranged from 2 to 5. The observed and expected heterozygosities were in the range of 0.024 to 0.561 and 0.024 to 0.535, respectively. Identifying genetic differences between populations, they are suitable markers for researches investigating genetic diversity and population structure of P. steinenii, which provide us with clues to dispersion, evolution and ecology of this species.

Keyword: Parochlus steinenii, Antarctica winged midge, microsatellite marker, next generation sequencing, evolution, population genetics