HOME LOG IN REGISTER
 
GENERAL INFORMATION
About the Journal
Editorial Board
Author Guidelines
Ethics
SUBMISSION & REVIEW
Authors
Reviewers
Associate Editors
Editor
Editor-In-Chief
Editorial Office
ARTICLES
Current Issue
Articles In Press
Search for Archives
Archive (Full Text)
Subject Keyword Abstract Author
 

e-ased.org, vol. 35, no. 4, pp.186-190, 2019

DOI: https://doi.org/10.5635/ASED.2019.35.4.023

Molecular Characterization of Two Marine Tintinnids (Ciliophora, Spirotrichea, Tintinnidae) Using Six Genes

Ji Hye Moon1, Atef Omar 2,3, Pablo Quintela-Alonso4, Jae-Ho Jung1,*

Affiliation: 1Department of Biology, Gangneung-Wonju National University, Gangneung 25457, Korea 2Natural Science Research Institute, Gangneung-Wonju National University, Gangneung 25457, Korea 3Department of Zoology, Al Azhar University, Assiut 71524, Egypt 4Department of Genetics, Physiology and Microbiology, Faculty of Biology, Complutense University of Madrid, Madrid 28040, Spain

Abstract: DNA barcoding of two marine tintinnids, Eutintinnus rectus and Schmidingerella arcuata, was performed using four samples collected from different sites in the north-eastern coast of South Korea. The loricae morphology was observed by light and scanning electron microscopy. Molecular data were analyzed using five nuclear ribosomal DNA markers (18S, ITS1, 5.8S, ITS2, and 28S genes) and one mitochondrial marker (CO1 gene). The intraspecific pairwise differences of E. rectus and S. arcuata in the CO1 gene were 0.0-0.2% and 0.0-0.6%, respectively, while there were no differences in the 18S rDNA sequences.

Keyword: CO1, DNA barcode, Eutintinnus rectus, planktonic ciliates, rDNA, Schmidingerella arcuata